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This laboratory manual outlines the procedures for the purification of an enzyme, ß-galactosidase, from a strain of E. coli cells. Extracting and purifying this protein from E. coli will be a multi-step process, starting from cell paste of E. Coli, progressing through several separation methods, and ending with analysis and verification of the purified protein by polyacrylamide gel electrophoresis (PAGE) and Immunoblotting procedures. The project is designed, as much as possible, to simulate a “real world” biotechnology experience in order to present the skills needed to do similar projects on the job.
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Date Issued 2005
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Rights Madison Area Technical College, Biotechnology Project
Date Of Record Creation 2008-05-14 09:46:37
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